A Multilayered Pdms Based Microtas for High-sensitivity Insulin Detection
نویسندگان
چکیده
This paper reports a multilayered, automated PDMS based microTAS for rapid, high-sensitivity and point-of-care insulin detection. Two generations of microTAS differing in their design complexity and insulin detection protocol were compared. The first generation microTAS includes one micropump, one micromixer and interdigitated electrodes to perform flow through impedance spectroscopy for insulin detection with a detection limit of 10 -5 M achieved within 20 min. The second generation microTAS includes multiple micropumps and valves, and is based on double antibody sandwich chemiluminescence immunoassay for insulin detection with a detection limit of 2.6 x 10 -10 M achieved within 10 min.
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